ABI Vii 7 Real Time PCR (General Facility Project No: GS 01/02)

Features:
• Optics: 6 emission filter (500-720 nm), 6 excitation filters (450-670 nm), OptiFlextm
System (enhanced flourescence detection).
• Temperature Range & Uniformity: 4-100°C, ±0.5°C, within 30 sec of reaching temprature
set point.
• Data Collection: Multiple ramp method detection formats provide more flexibilty for
collecting dat during a ramp stage.
• Dynamic Range: Up to 9 logs of linear dynamic range.
• Detection Method: Primer - Probe Detection, SYBR.
• Precise Quantification: Detect changes as small as 1.5-fold in target quantities in
singleplex reactions.
• Run Time: About 35 min. (Varies depending on reagents and other factors).

Applications:
• Absolute quantitation (standard curve).
• Relative quatitation (ΔΔCt or Comparativve Ct).
• SYBR green assay.
• Allelic discrimination (SNP Genotyping).
• Plus Minus Assay (Presence/ Absence).
• Qualitative Analysis.
• High Resolution Melting (HRM) analysis.

Sample Analysis Request Form

Analysis Request form Real Time PCR 

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